Genomics and Sequencing Core
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Main Contact Info
David Looney
Remittance Contact Info
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The following Products and Services are available within our facility:
INSTRUMENT TIME
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Microarray printerINST-001: Microarray Printer |
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HPAEC-PADINST-002: HPAEC-PAD |
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GCMSINST-003: GC-MS |
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HPAEC-UVINST-004: HPAEC-UC |
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HPLCINST-005: HPLC |
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HPLC-FLINST-006: HPLC-FL |
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UPLC-FLINST-007: UPLC-FL |
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SpeedVacINST-008: SpeedVac |
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MALDI-QTOFINST-009: LTQ-MS |
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LTQ-MSINST-010: LTQ-MS |
PREPARATIONS
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Mixed N-glycan preparationPRE-001: Mixed N-glycan preparation |
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Mixed O-glycan preparationPRE-002: Mixed O-glycan preparation |
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Glycosaminoglycan preparationPRE-006: Glycosaminoglycan preparation |
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Extraction of semi-rough or smooth LPSPRE-009: Extraction of semi-rough or smooth LPS |
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Capsular polysaccharide extractionPRE-011: Capsular polysaccharide extraction |
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Sugar nucleotide extractionPRE-013: Sugar nucleotide extraction |
MODIFICATIONS
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Alditol acetate (AA) derivative for GC-MSMOD-001: Alditol acetate (AA) derivative for GC-MS |
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Trimethyl silyl (TMS) derivative for GC-MSMOD-002: Trimethyl silyl (TMS) derivative for GC-MS |
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Per-O-methylation (PM) of glycansMOD-003a: Per-O-methylation (PM) of glycans |
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Partially methylated alditol acetate (PMAA)MOD-003b: Partially methylated alditol acetate (PMAA) |
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Fatty acid methyl ester (FAME)MOD-004: Fatty acid methyl ester (FAME) |
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Mild acid treatment for releasing sialic acidMOD-005: Mild acid treatment for releasing sialic acid |
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De-O-acetylation of glycansMOD-006: De-O-acetylation of glycans |
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Enzymatic degradation of chondroitin sulfate (GAG-CS)MOD-007: Enzymatic degradation of chondroitin sulfate (GAG-CS) |
Enzymatic degradation of heparan sulfate and heparin (GAG-HS) |
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Isotopic aniline tagging for GAG disaccharide analysis by GRIL-MSMOD-009: Isotopic aniline tagging for GAG disaccharide analysis by GRIL-MS |
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2-AB labeling of glycansMOD-010: 2-Aminobenzamide (2-AB) labeling of glycans |
COMPOSITIONS
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Monosaccharide analysis by HPAEC-PADCOM-001: Monosaccharide analysis by HPAEC-PAD |
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Uronic acid analysis by HPAEC-PADCOM-001: Monosaccharide analysis by HPAEC-PAD |
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Sialic acid analysis by HPAEC-PADCOM-003: Sialic acid analysis by HPAEC-PAD |
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RP HPLC of DMB-sialic acid (determination by fluorescence detector)COM-005: RP-HPLC of DMB-sialic acid (determination by fluorescence) |
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GC-MS of TMS derivativeCOM-006: GC-MS of TMS derivative |
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GC-MS of AA derivativeCOM-007: GC-MS of AA derivative |
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GC-MS of PMAA derivative (linkage analysis)COM-008: GC-MS of PMAA derivative (linkage analysis) |
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GC-MS of fatty acidsCOM-009: GC-MS of fatty acids |
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LTQ-MS (per hour; single run, no sample prep)COM-010: LTQ Orbitrap-MS (single hour run run, no sample prep) |
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LTQ-MS (per 20 min; single run, no sample prep)COM-010a: LTQ Orbitrap-MS (single 20 min run, no sample prep) |
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Glycan analysis by LTQ-MSCOM-011: Glycan analysis by LTQ-MS |
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Nucleotide sugar analysis by LCMSCOM-012: Nucleotide sugar analysis by HPAEC-UV |
PROFILINGS
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O-glycan profiling by HPAEC-PADPRO-002: O-Glycan profiling by HPAEC-PAD |
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2-AB-labeled glycan profiling by HPAEC-FLPRO-003: 2-AB-labeled glycan profiling by HPAEC-FL |
HPLC (per hour; single run, no sample prep) |
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MALDI-TOF profiling of glycansPRO-007: MALDI-TOF profiling of glycans |
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Cellular metabolite analysis by HPAEC-UVPRO-008: Cellular metabolite analysis |
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GAG disaccharide analysis by GRIL LTQ-MSPRO-009: GAG disaccharide analysis by GRIL LTQ-MS |
MISCELLANEOUS
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Consultation time (per hour)MISC-001: Consultation time (per hour) |
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Methods development (per hour, plus materials)MISC-003: Methods development (per hour) |
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Training (per hour)MISC-007: Training (per hour) |
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Training – visiting (per day)MISC-007a: Training (per day; visitor) |
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Additional Staff time techMISC-008: Additional Staff Time Tech |
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