Illumina NextSeq 2000 sequencer is offered for assisted self-service. You can provide a library pool and a flow cell kit. Alternatively, you can order a flow cell kit via the core. Three output sizes are available: P1, P2, and P3 producing 100 million, 400 million, or 1 billion reads (or read pairs) per run respectively. P1 is available only as a 300-cycle kit. P2 and P3 are available as 100, 200, or 300-cycle kits. Select the flow cell you will use when booking a time on the NextSeq 2000.

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The Dragonfly 200 is a high-speed confocal imaging platform that offers confocal, laser-based widefield, transmitted light and SRRF-Stream+ functionality in one system. The system is designed to deliver high-quality images even in samples with weak staining. Confocal speeds of up to 400 fps, Borealis enhanced illumination for a large and flat field of view, ClearView-GPU deconvolution, Dual-camera acquisition, Choice of optical zoom and pinhole size

Chromium Single Cell Gene Expression provides single cell transcriptome 3' gene expression and multiomic capabilities to profile tens of thousands of cells. Explore cellular heterogeneity, novel targets, and biomarkers with combined gene expression, surface protein expression, or CRISPR edits in each cell. Charged by sample. This price includes all necessary reagents and QC checks.

Assisted use of GeoMx DSP NanoString is offered. The GeoMx Digital Spatial Profiler (DSP) is a high-throughput spatial profiling platform that enables researchers to simultaneously analyze RNA and protein targets in a wide range of sample types, including FFPE tissue sections, fresh frozen tissue sections, and cell suspensions. The platform is designed to provide highly multiplexed spatial profiling with high sensitivity and specificity.

Fully automated and easy-to-use, the nCounter MAX Analysis System provides everything you need to complete your projects in record time. Faster and highly multiplexed than qPCR and simpler than NGS, the nCounter MAX allows for digital examination of multiple pathways from a single tube in an extraction-free workflow requiring only 15 minutes of hands-on time. Accelerate your research by spending less time on sample prep and perform your own data analysis using the included nSolver™ Analysis Software.

IMARIS training videos https://bit.ly/imaris3

A software suite that can help researchers extract quantitative information from images and perform statistical analysis on the data. It is designed to help you visualize, analyze, segment and interpret 3D and 4D microscopy datasets. The software can be used for a wide range of applications including cell biology, developmental biology, neuroscience, and more. Some of the key features of Imaris include visualization of large datasets in 3D and 4D, automated image analysis and segmentation, quantitative measurements of objects and structures, statistical analysis of data, and creation of publication-quality images and movies.

The Olympus FLUOVIEW FV1000 confocal laser scanning microscope delivers efficient and reliable performance together with the high resolution required for multi-dimensional observation of cell and tissue morphology, and precise molecular localization. The FV1000 incorporates the industry’s first dedicated laser light stimulation scanner to achieve simultaneous targeted laser stimulation and imaging for real-time visualization of rapid cell responses. The FV1000 also measures diffusion coefficients of intracellular molecules, quantifying molecular kinetics. Quite simply, the FLUOVIEW FV1000 represents a new plateau, bringing “imaging to analysis.”

The Zeiss LSM 780 is a multi-purpose, visible light confocal laser scanning microscope. It is equipped with a 34 channel spectral array with laser lines at 405nm, 458nm, 488nm, 514nm, 561nm, 594nm and 633nm.The microscope is inverted and is outfitted with a full incubation chamber for regulating temperature and CO² for live cell imaging. The microscope also has a motorized stage and software for tile imaging which allows extremely high resolution files from large fields of view.

It has been moved to the flow core, please reserve it there.

The E220 Focused-ultrasonicator enables multi-sample batch preparation, capable of processing a wide range of sample types and volumes. The E220 may be programed to process from 1 to 96 samples in a single batch.

An all-in-one fluorescence microscope that captures clear images with full electronic control and no darkroom required. This microscope greatly changes the conventional wisdom of microscopy such as the observation of communication processes between live cells, the high-resolution observation of whole targets, and the understanding of dispersion while viewing the full profile.

The Keyence BZ-X800 is a versatile, all-in-one fluorescence microscope designed for high-resolution imaging and advanced analysis. It supports live imaging and accommodates multi-well plates, enabling efficient high-throughput studies. The microscope features four-color fluorescence, bright field imaging, tiling, and stitching for comprehensive sample coverage. It offers 3D views using structured illumination, allowing for precise z-stack imaging. This makes it ideal for detailed visualization of complex cellular structures and biological samples, suitable for a wide range of research applications from basic cell biology to advanced biomedical studies.

The MiSeq System offers the first DNA-to-data sequencing platform integrating cluster generation, amplification, sequencing, and data analysis into a single instrument. There is a $200 recharge rate for a MiSeq run.

Thermo Scientific™ NanoDrop 2000 and 2000c are full-spectrum, UV-Vis spectrophotometers used to quantify and assess purity of DNA, RNA, Protein and more. The NanoDrop 2000 and 2000c are the only microvolume spectrophotometers with patented sample retention technology that measure sample volumes as small as 0.5μL.

The Applied Biosystems ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems thermal cyclers with the flexible configuration and control features that fit how you work today. Interchangeable block formats allow you to maximize your throughput or run independent experiments concurrently.

The Invitrogen Qubit Fluorometers were developed to work optimally with the Qubit Assays. Together they quickly and specifically quantify DNA, RNA, and protein. It is easier than ever to determine if you have sufficient nucleic acid or protein for your experiment. The price includes all necessary assay components. High Sensitivity DNA and High Sensitivity RNA assays are stocked by the Genomics Core

The Agilent TapeStation 4200 system is an automated electrophoresis solution for the sample quality control of DNA and RNA samples. The system integrates an instrument, data processing software, reagents, and ScreenTape devices specific for DNA and RNA. It is suitable to analyze size, quantity, and integrity of your samples. It fits for example in a next-generation sequencing (NGS) or biobanking workflow with low to high throughput delivering highly precise analytical evaluation. The TapeStation 4200 is capable of running up to 96 samples in a single run, allowing for a higher throughput than ever before. High Sensitivity D1000, High Sensitivity D5000, High Sensitivity RNA, and Genomic DNA screen tapes and reagents are stocked by the core. Their use is included in the order price.

The C1000 Touch PCR thermal cycler offers superior performance and a color touch screen for easy programming. It can accommodate different throughput needs with easily interchangeable reaction modules that swap in seconds.

Reservation to use the bench in B007. This reservation is not required if you have booked time on one of the instruments in B007. Reservation includes the use of pipettes, pipette tips, tubes, and PCR machines. This reservation does not include the use of any other equipment.

Microtome is used for cutting paraffin blocks to produce tissue sections for staining and microscopy. The microtome is a precision instrument that poses a significant risk of injury, including severe cuts. Users must complete proper training before operating the device. Use of the microtome is governed by UCSD and SCRM safety policies, and users are required to follow these protocols at all times. This equipment is provided by the Genomics Core for unassisted use only by trained personnel.

In the event of an injury, immediately stop work and apply first aid to control bleeding. Seek medical attention if necessary, and report the incident to your supervisor. Notify the UCSD Environment, Health & Safety (EH&S) office promptly, and ensure the injury is documented as per UCSD and SCRM safety protocols. Emergency contact information and first aid kits are available in the lab.

Tanner TN8000 microtome

The Vibratome Leica VT1000S is a tissue sectioning instrument designed for precise cutting of soft biological specimens. It operates with a vibrating blade, minimizing tissue deformation during sectioning. The system supports sectioning for both fixed and live tissues, allowing the latter to be cut for further cultivation. Adjustable cutting parameters, such as amplitude and speed, accommodate different sample types. This vibratome is commonly used in neurobiology, histology, and molecular biology research.
The vibratome is a precision cutting instrument that carries a risk of injury, including cuts. Users must complete proper training before operating the device. The use of the vibratome is subject to UCSD and SCRM safety policies, and adherence to these protocols is mandatory. This equipment is provided by the Genomics Core for unassisted use only by trained personnel.

In case of injury, immediately stop work and administer first aid to control bleeding. If needed, seek medical attention and report the incident to your supervisor. Promptly notify the UCSD Environment, Health & Safety (EH&S) office, and ensure the injury is documented in accordance with UCSD and SCRM safety guidelines. Emergency contact information and first aid supplies are readily available in the lab.